Question

Transposons can be used during which one of the following?

• A. Polymerase chain reaction
• B. Gene silencing
• C. Autoradiography
• D. Gene sequencing

Answer 1

The Correct Option is D

Transposons, often referred to as "jumping genes," are sequences of DNA that can change their position within the genome. This ability to move and insert themselves into new locations makes transposons useful tools in genetic research and biotechnology, particularly in the field of gene sequencing.

During gene sequencing, transposons can serve various purposes such as:

1. Mutagenesis: Transposons can be used to disrupt genes, creating mutations that help in identifying gene functions. By inserting into different parts of the genome, transposons can 'tag' genes, making it easier to locate and sequence them.
2. Mapping and Annotation: The random insertion of transposons enables researchers to create detailed maps of genomes, facilitating the annotation of genes and regulatory elements. This helps in understanding the organization and function of genetic material.
3. Sequencing Techniques: Specific transposizable elements can be employed in next-generation sequencing techniques. They can help fragment DNA into smaller, manageable sections which can then be sequenced and assembled to understand the complete nucleotide sequence of an organism’s DNA.

Thus, among the given options, transposons are most relevant to gene sequencing due to their capability to facilitate genomic research and data acquisition.