Question:
There is a restriction endonuclease called $EcoRI$. What does '' $co$'' part in it stand for ?
There is a restriction endonuclease called $EcoRI$. What does '' $co$'' part in it stand for ?
Updated On: Aug 1, 2022
- Coenzyme
- coli
- Colon
- Coelom
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The Correct Option is B
Solution and Explanation
The enzyme restriction endonuclease EcoRI is found in the colon bacteria E. coli. So, here 'co' stands for coli. According to nomenclature of restriction enzyme, the first letter used for the enzyme is the first letter of the genus name (in italics) of the bacterium, then comes the first two letters of its species (also in italics), next is the strain of the organism. At last is a Roman numeral signifying the order of discovery. Here, the enzyme EcoRl was isolated from the bacterium Escherichia coli (co), strain RY13(R) and it was first endonuclease (I) isolated from E.coli.
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
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- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.