The taq polymerase enzyme is obtained from
- Thiobacillus ferroxidans
- Bacillus subtilis
- Pseudomonas putida
- Thermus aquaticus
The Correct Option is D
Solution and Explanation
The correct answer is:
Option 4: Thermus aquaticus
Explanation:
Taq polymerase is a heat-stable enzyme that is commonly used in the Polymerase Chain Reaction (PCR). It is isolated from the thermophilic bacterium Thermus aquaticus, which thrives in hot environments like hot springs. This enzyme can withstand the high temperatures required during the denaturation step of PCR, making it invaluable for the process of amplifying DNA.
Why Other Options Are Incorrect:
Option 1: Thiobacillus ferroxidans – This is a bacterium involved in sulfur oxidation, not used for Taq polymerase production.
Option 2: Bacillus subtilis – A common bacterium used in biotechnology, but it does not produce Taq polymerase.
Option 3: Pseudomonas putida – Known for its versatility in degrading organic compounds, but it is not the source of Taq polymerase.
Thus, Taq polymerase is obtained from Thermus aquaticus, a thermophilic bacterium.
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.