Question

The restriction map of a circular plasmid is shown below, along with the indicated distances between the restriction sites.

The plasmid was completely digested with EcoRI, and XhoI. The products were analysed by agarose gel electrophoresis followed by ethidium bromide staining. The number of bands that will be visible in the gel when exposed to UV light is __________.

Answer 1

Correct Answer: 3

EcoRI cuts the plasmid into two fragments (2 kb and 3 kb), and XhoI cuts it into 2 fragments (2 kb and 5 kb). The restriction map shows two EcoRI and two XhoI sites, leading to a total of three distinct bands when the plasmid is completely digested