Question

The principle of fluorescence microscopy is based on:
1. A substance that causes the rotation of polarised light
2. Differences in the optical light path being converted to intensity differences
3. Differences in refractive index being converted into variations in light intensity
4. The absorption of light and its subsequent emission at a longer wavelength

• A. A substance that causes the rotation of polarized light
• B. Differences in the optical light path being converted to intensity differences
• C. Differences in refractive index being converted into variations in light intensity
• D. The absorption of light and its subsequent emission at a longer wavelength

Hint:

Fluorescence microscopy is widely used in biological sciences to study specific structures like proteins, DNA, or organelles by tagging them with fluorescent markers.

Answer 1

The Correct Option is D

Fluorescence microscopy is based on the principle that certain substances absorb light (usually ultraviolet or blue light) at one wavelength and then emit it at a longer wavelength (visible light). This property of fluorescence allows specific structures or molecules in a specimen to be tagged with fluorescent dyes, enabling detailed visualization.