To determine the factor by which the de Broglie wavelength of an electron changes when the potential difference used to accelerate it is quadrupled, we start with the relevant formulae. The de Broglie wavelength λ is given by:
$$ \lambda = \frac{h}{p} $$
where \( h \) is Planck's constant and \( p \) is the momentum of the electron.
The momentum \( p \) can be expressed in terms of the kinetic energy \( K \) as:
$$ p = \sqrt{2mK} $$
The kinetic energy \( K \) acquired by the electron is due to the potential difference \( V \), so \( K = eV \), where \( e \) is the charge of the electron.
Thus, the momentum becomes:
$$ p = \sqrt{2meV} $$
Substitute this expression for \( p \) into the de Broglie wavelength formula:
$$ \lambda = \frac{h}{\sqrt{2meV}} $$
If the potential difference \( V \) is quadrupled, the new wavelength \( \lambda' \) when the potential is \( 4V \) is:
$$ \lambda' = \frac{h}{\sqrt{2m \cdot 4eV}} $$
Simplifying the expression gives:
$$ \lambda' = \frac{h}{2\sqrt{2meV}} $$
Now, compare \( \lambda' \) with the original \( \lambda \):
$$ \frac{\lambda'}{\lambda} = \frac{\frac{h}{2\sqrt{2meV}}}{\frac{h}{\sqrt{2meV}}} = \frac{1}{2} $$
This means the de Broglie wavelength is halved. The factor by which the wavelength changes is 0.5.

Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 