An electron is accelerated from rest through a potential difference of 200 V. To find the de Broglie wavelength associated with this electron, we apply the following steps:
1. Kinetic Energy (KE): When an electron is accelerated through a potential difference \(V\), it gains kinetic energy equal to the work done by the electric field:
\(KE = eV\), where \(e = 1.6 \times 10^{-19} \text{ J} \text{ (charge of electron)}\).
2. Calculating KE: Here, \(V = 200 \text{ V}\), so:
\(KE = 1.6 \times 10^{-19} \times 200 = 3.2 \times 10^{-17} \text{ J}\).
3. Relating KE to momentum: The kinetic energy of the electron also relates to its momentum \(p\) by the formula:
\(KE = \frac{p^2}{2m}\), where \(m = 9.1 \times 10^{-31} \text{ kg} \text{ (mass of electron)}\).
4. Solving for momentum (p): By rearranging, \(p = \sqrt{2m \cdot KE}\):
\(p = \sqrt{2 \times 9.1 \times 10^{-31} \times 3.2 \times 10^{-17}}\)
\(p = \sqrt{5.824 \times 10^{-47}}\)
\(p = 7.63 \times 10^{-24} \text{ kg m/s}\).
5. Applying de Broglie wavelength formula: The de Broglie wavelength \(\lambda\) is given by:
\(\lambda = \frac{h}{p}\), where \(h = 6.6 \times 10^{-34} \text{ J s}\) (Planck's constant).
6. Calculating wavelength: \(\lambda = \frac{6.6 \times 10^{-34}}{7.63 \times 10^{-24}}\)
\(\lambda = 8.65 \times 10^{-11} \text{ m}\).
7. Converting meters to nanometers: \(1 \text{ nm} = 10^{-9} \text{ m}\), so:
\(\lambda = 0.0865 \text{ nm}\)
8. Rounding: The wavelength is \(0.09 \text{ nm}\) to 2 decimal places.
Verification: The calculated de Broglie wavelength \(0.09 \text{ nm}\).

Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 