Match the following:
List-1: List-2:
A Physical barrier I Lysozyme
B Physiological barriers II Interferons
C Cellular barriers III Monocytes
D Cytokine barriers IV Colostrum
V Mucus membrane
A shopkeeper buys an item for Rs 2000 and marks it up by 50% to set the marked price. He then offers a 20% discount on the marked price. What is the profit earned by the shopkeeper?