The linking of the antibiotic resistance gene with the plasmid vector of Salmonella typhimurium by Stanley Cohen and Herbert Boyer was made possible by the enzyme:
DNA ligase is used to join the antibiotic resistance gene with the plasmid vector.
- This technique was a pioneering step in recombinant DNA technology.
- DNA ligase is used to join the antibiotic resistance gene with the plasmid vector.
- This technique was a pioneering step in recombinant DNA technology.

| S. No. | Particulars | Amount (in ₹ crore) |
|---|---|---|
| (i) | Operating Surplus | 3,740 |
| (ii) | Increase in unsold stock | 600 |
| (iii) | Sales | 10,625 |
| (iv) | Purchase of raw materials | 2,625 |
| (v) | Consumption of fixed capital | 500 |
| (vi) | Subsidies | 400 |
| (vii) | Indirect taxes | 1,200 |