Question:
The given figure is the diagrammatic representation of the E. coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)?
The given figure is the diagrammatic representation of the E. coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)?
Updated On: Jul 29, 2022
- ori-original restriction enzyme
- rop-reduced osmotic pressure
- Hind III, EcoRI - selectable markers
- amp $^R$, tet $^R$- antibiotic resistance genes
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The Correct Option is D
Solution and Explanation
In pBR 322, ori-represents site or origin of replication rop-codes for proteins that take part in the replication of plasmid. Hin d III, Eco RI- recognition sites of restriction endonucleases. amp and tet - antibiotic resistance genes.
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
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