Question

The following diagram shown restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’gens :

• A. The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid.
• B. The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.
• C. The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics
• D. Gene ’X’ is responsible for recognitions sites and ‘Y’ is responsible for antibiotic resistance

Answer 1

The Correct Option is B

The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.

‘X’ in the given diagram is ori while ‘Y’ is rop.

‘X’ which is ori is responsible for controlling the copy number of the linked DNA and ‘Y’ which is rop codes for protein involved in the replication of plasmid.

Options (1), (3) and (4) are incorrect as ‘X’ and ‘Y’ are not related to these functions.