The first recombinant DNA was constructed by linking an antibiotic resistant gene with the native plasmid of
- Escherichia coli
- Salmonella typhimurium
- Clostridium butylicum
- Acetobacter aceti
The Correct Option is B
Solution and Explanation
Top Questions on recombinant technology
- Molecular scissor of genetic engineering?
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- 0.16 seconds is a time interval for which event in the ECG system?
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Frequency of recombination between gene pairs on same chromosome as a measure of the distance between genes to map their position on chromosome, was used for the first time by
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- Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
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- During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
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Questions Asked in KEAM exam
- In the measurement of length 6 $\mu$m is equal to $x$ pm. Then the value of $x$ is
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- Dimensional Analysis
- If the lines \( \frac{x-1}{2} = \frac{y-2}{\alpha} = \frac{z-3}{2} \) and \( \frac{x-1}{2} = \frac{y-2}{1} = \frac{z-3}{-2} \) are perpendicular, then the value of \( \alpha \) is:
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- Integration
- An organic compound contains 37.5% C, 12.5% H and the rest oxygen. What is the empirical formula of the compound?
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- Oxygen
- Let A, B, C denote the set of students in a college who play football, basketball, and cricket respectively. If \( n(A) = 60 \), \( n(B) = 55 \), \( n(C) = 70 \), \( n(A \cup B \cup C) = 100 \) and \( n(A \cap B \cap C) = 20 \), then the number of students who play exactly two of these sports is
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- Relations and functions
For the reaction:
\[ 2A + B \rightarrow 2C + D \]
The following kinetic data were obtained for three different experiments performed at the same temperature:
\[ \begin{array}{|c|c|c|c|} \hline \text{Experiment} & [A]_0 \, (\text{M}) & [B]_0 \, (\text{M}) & \text{Initial rate} \, (\text{M/s}) \\ \hline I & 0.10 & 0.10 & 0.10 \\ II & 0.20 & 0.10 & 0.40 \\ III & 0.20 & 0.20 & 0.40 \\ \hline \end{array} \]
The total order and order in [B] for the reaction are respectively:
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- Grignard’s reagents and their uses
Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.