Question

The enriched media used to facilitate the growth of fastidious microorganisms are

• A. Selenite F broth
• B. Blood agar
• C. Chocolate agar
• D. Loeffler's serum

Answer 1

The Correct Option is B, C, D

In microbiology, certain microorganisms, known as fastidious organisms, require specific nutrients and conditions for growth. Enriched media are specially formulated to support the growth of such organisms by providing the essential nutrients they need.

Let's examine each option:

• Selenite F broth: This is primarily used as an enrichment medium for the isolation of Salmonella species from feces. It is not specifically designed for fastidious organisms.
• Blood agar: This is an enriched medium that contains general nutrients and 5-10% sheep, rabbit, or horse blood. It supports the growth of fastidious bacteria and is helpful in determining hemolytic reactions. It promotes the growth of a variety of pathogens.
• Chocolate agar: A form of blood agar in which the blood is lysed, providing additional nutrients such as NAD and hemin, which are important for the growth of certain fastidious organisms like Haemophilus influenzae and Neisseria species.
• Loeffler's serum: This medium supports the growth of fastidious bacteria such as Corynebacterium diphtheriae. It enriches with serum, providing necessary growth factors.

Given these descriptions, the correct enriched media for the growth of fastidious microorganisms are:

• Blood agar
• Chocolate agar
• Loeffler's serum

These media contain the required nutrients and conditions that facilitate the growth of various fastidious organisms by providing additional growth factors and nutrients.