The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bacteria because of
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Alpha-galactosidase catalyzes the hydrolysis of glycolipids and glycoproteins . It is a glycoside hydrolase enzyme that hydrolyzes from its terminal alpha-galactosyl moieties. Insertional inactivation is used to classify the transformed bacterial cells that have taken recombinant plasmids in bacterial DNA technology.
- Inactivation of glycosidase enzyme in recombinant bacteria
- Non-recombinant bacteria containing $\beta$-galactosidase
- Insertional inactivation of alpha-galactosidase in nonrecombinant bacteria
- Insertional inactivation of $\alpha$-galactosidase in recombinant bacteria
The Correct Option is B
Solution and Explanation
The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bacteria because of non-recombinant bacteria containing \(\beta\)-galactosidase.
In cloning experiments, blue-white screening is done to detect recombinant bacteria. α-complementation plays a major role evaluation of the recombinant nature of the bacteria.
- In normal cells, blue colonies are formed when β-galactosidase is active.
- In recombinant cells, α-complementation does not occur. No blue color is noted.
- It is due to the insertional inactivation of alpha-galactosidase in recombinant bacteria that the colonies of recombinant bacteria appear white in comparison to the blue colonies of non-recombinant bacteria.
Also Read: Bacterial Transformation
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.