Question:

The best and latest technique for isolation, purification and separation of organic compounds is

Updated On: Jan 20, 2026
  • Crystallisation

  • Distillation

  • Sublimation

  • Chromatography

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The Correct Option is D

Solution and Explanation

To determine the best and latest technique for the isolation, purification, and separation of organic compounds, let's analyze each of the given options:

  • Crystallisation: This method involves dissolving a substance in a hot solvent and then cooling it slowly to form crystals. While it's effective for purification, it's not suitable for separating complex mixtures of organic compounds.
  • Distillation: This technique is used to separate components based on their boiling points. It works well for liquids but is less effective for complex organic mixtures, especially those with similar boiling points.
  • Sublimation: Involves converting a solid directly into a gas and then back into a solid. This method is limited to compounds that can sublimate and is not versatile for general organic compound separation.
  • Chromatography: This is a broad range of techniques where components of a mixture are separated based on their movement through a medium. Chromatography is highly effective for separating complex mixtures and is widely used for both qualitative and quantitative analysis of organic compounds.

Based on the above analysis, Chromatography is the most suitable and advanced technique for the isolation, purification, and separation of organic compounds. This conclusion is made due to its versatility, precision, and efficacy in handling complex mixtures. Chromatography is instrumental in modern chemical laboratories due to its ability to separate components with high resolution and sensitivity.

Thus, the correct answer is Chromatography.

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Concepts Used:

Organic Chemistry – Some Basic Principles and Techniques - Reaction Mechanism

SN1 Reaction Mechanism:

SN1 reaction mechanism takes place by following three steps –

  • Formation of carbocation 
  • Attack of nucleophile 
  • Deprotonation 

SN2 Reaction Mechanism:

The SN2 reaction mechanism involves the nucleophilic substitution reaction of the leaving group (which generally consists of halide groups or other electron-withdrawing groups) with a nucleophile in a given organic compound.

Electrophilic Aromatic Substitution Reaction Mechanism:

The mechanism of an electrophilic aromatic substitution reaction contains three main components which are:

  1. A new sigma bond from C=C is formed during the reaction in the arene nucleophile.
  2. Due to the breaking of the C-H sigma bond, a proton is removed.
  3. The C=C bond is reformed and it restores the aromaticity of the compound.

Electrophilic Substitution Reaction Mechanism:

The electrophilic substitution reaction mechanism is composed of three steps, which will be discussed more below.

  • Electrophile Generation
  • Carbocation Formation
  • Proton Removal