Question

Tetrapeptide(s) that gives(give) the following product on reaction with Sanger's reagent followed by hydrolysis is(are)
\includegraphics[width=0.5\linewidth]{38.jpeg}

• A. Ala-Gly-Leu-Phe
• B. Asp-Phe-Leu-Pro
• C. Asp-Gly-Tyr-Phe
• D. Ala-Phe-Tyr-Pro

Hint:

Sanger's reagent reacts with the N-terminal amine group of peptides to identify the first amino acid in the sequence. Hydrolysis then breaks down the peptide bond.

Answer 1

The Correct Option is A, D

Step 1: Understanding the Sanger's reagent reaction.
Sanger's reagent reacts with the free amine group in peptides and proteins, allowing for the identification of the N-terminal amino acid. Upon hydrolysis, the peptide bond breaks, releasing the individual amino acids. The structure provided corresponds to the expected product after Sanger's reaction.

Step 2: Analyzing the options.
(A) Ala-Gly-Leu-Phe: Incorrect — The reaction would not yield this product as shown.
(B) Asp-Phe-Leu-Pro: Incorrect — This does not match the expected structure after reaction and hydrolysis.
(C) Asp-Gly-Tyr-Phe: Correct — This matches the expected product of the Sanger's reagent reaction and hydrolysis.
(D) Ala-Phe-Tyr-Pro: Incorrect — This structure does not result from the described reaction.

Step 3: Conclusion.
The correct answer is (C) as it matches the expected product after reaction with Sanger's reagent and hydrolysis.