Suppose DNA samples collected for DNA fingerprinting analysis are less than the required quantity. Which of the following techniques is helpful to make the samples sufficient for above analysis?
- Electrophoresis
- Chromatography
- PCR
- DNA probing
The Correct Option is C
Approach Solution - 1
PCR (Polymerase Chain Reaction) is a technique used to amplify (make millions of copies of) a small amount of DNA, making it sufficient for analysis like DNA fingerprinting.
- (A) Electrophoresis: Separates DNA fragments by size but does not amplify DNA.
- (B) Chromatography: Separates molecules based on chemical properties but cannot replicate DNA.
- (D) DNA probing: Identifies specific DNA sequences using labeled probes but does not increase DNA quantity.
Thus, PCR (C) is the only technique that solves the problem of insufficient DNA samples.
Approach Solution -2
PCR (Polymerase Chain Reaction): Amplifies tiny DNA samples exponentially.
Other techniques:
- (A) Electrophoresis: Separates DNA by size.
- (B) Chromatography: Separates molecules by affinity.
- (D) DNA probing: Detects specific sequences.
The correct answer is (C) PCR.
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