To identify Cryptococcus in microbiological samples, a specific staining technique is used. Let's evaluate the provided options:
- ZN stain: Also known as Ziehl-Neelsen stain, is commonly used for acid-fast bacteria like Mycobacterium tuberculosis, not Cryptococcus.
- Gram stain: While this can stain Cryptococcus, it is not specific for this microorganism as it is more generalized for differentiating bacteria.
- Mucicarmine stain: Specifically binds to the polysaccharide capsule of Cryptococcus, providing a clear identification due to their characteristic red color against a blue background.
- Malachite green: Typically used for spore staining in bacteria, not for Cryptococcus.
Given these evaluations, the correct staining method specifically utilized for identifying Cryptococcus is the Mucicarmine stain.