Question:

Select the correct sequence of steps in DNA fingerprinting involving southern blot hybridization using radiolabelled VNTR as a probe. I. $\quad$Hybridization using labelled VNTR probe. II. $\quad$Isolation of DNA. III. $\quad$Transferring (blotting) of separated DNA fragments to synthetic $\quad$$\quad$membranes, such as nitrocellulose or nylon. IV.$\quad$ Detection of hybridized DNA fragments by autoradiography. V. $\quad$Separation of DNA fragments by electrophoresis. VI. $\quad$Digestion of DNA by restriction endonucleases.

Updated On: Jul 28, 2022
  • I, V, VI, II, III and IV
  • II, VI, V, IJI, I and IV
  • V, I, VI, III, IV and II
  • II, I, V, VI, IV and III
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The Correct Option is B

Solution and Explanation

Answer (b) II, VI, V, IJI, I and IV
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Concepts Used:

DNA Fingerprinting

​​DNA fingerprinting, also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing, in genetics, method of isolating and identifying variable elements within the base-pair sequence of DNA.

The procedure of DNA Fingerprinting:

The procedure for creating a DNA fingerprint consists of first obtaining a sample of cells, such as skin, hair, or blood cells, which contain DNA. The DNA is extracted from the cells and purified. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific points along the strand with proteins known as restriction enzymes. The enzymes produced fragments of varying lengths that were sorted by placing them on a gel and then subjecting the gel to an electric current (electrophoresis): the shorter the fragment, the more quickly it moved toward the positive pole (anode). The sorted double-stranded DNA fragments were then subjected to a blotting technique in which they were split into single strands and transferred to a nylon sheet. The fragments underwent autoradiography in which they were exposed to DNA probes—pieces of synthetic DNA that were made radioactive and that bound to the minisatellites. A piece of X-ray film was then exposed to the fragments, and a dark mark was produced at any point where a radioactive probe had become attached. The resultant pattern of marks could then be analyzed.