Question

Reverse Transcriptase Polymerase Chain Reaction is an analytical procedure used in detection of pathogenic microorganisms. Which one of the following statements is NOT correct in this context?

• A. It is used for identifying presence or absence of specific RNA in samples.
• B. It is used only for identifying Corona Viruses, including SARS CoV2 in samples.
• C. It cannot differentiate between viable and inactivated viruses.
• D. It is based on conversion of RNA to DNA followed by DNA amplification.

Hint:

RT-PCR detects genetic material and works for many RNA viruses—not only SARS-CoV-2.

Answer 1

The Correct Option is B

Reverse Transcriptase PCR (RT-PCR) is a molecular diagnostic technique used for detecting RNA-based pathogens. It works by converting RNA into complementary DNA (cDNA) followed by amplification.
Step 1: Understanding RT-PCR usage.
RT-PCR is widely used for detecting a variety of RNA viruses, such as influenza, HIV, hepatitis viruses, and SARS-CoV-2. It is \textit{not} specific only to coronavirus detection.
Step 2: Evaluate options.
Option (A) is correct — RT-PCR detects specific RNA sequences.
Option (C) is correct — RT-PCR cannot distinguish live vs. dead viruses.
Option (D) is correct — the process is RNA → cDNA → amplification.
Thus, (B) is the only incorrect (NOT correct) statement.
Final Answer: (B)