Question:
Restriction endonucleases are enzymes which
Restriction endonucleases are enzymes which
Updated On: Jul 28, 2022
- make cuts at specific positions within the DNA molecule
- recognize a specific nucleotide sequence for binding of DNA ligase
- restrict the action of the enzyme DNA polymerase
- remove nucleotides from the ends of the DNA molecule.
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The Correct Option is A
Solution and Explanation
Restriction endonucleases were found by Arber in 1962 in bacteria. They act as 'molecular scissors' or chemical scalpels. They recognize the specific base sequence at palindrome sites in DNA duplex and cut its strands. For example, restriction endonuclease EcoRX found in the colon bacteria E. coli reocgnizes the base sequence GAATTC in DNA duplex and cuts its strands between G and A.
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.