Ultracentrifugation separates molecules like proteins and nucleic acids based on density differences. Gel electrophoresis works on molecular mobility in an electric field. Gel filtration separates molecules by size using porous beads.
For the coupled reactions given below:
Glucose 6-phosphate + H\(_2\)O → Glucose + Pi (Reaction 1)
ATP + Glucose → ADP + Glucose 6-phosphate (Reaction 2) The standard free energy change of ATP hydrolysis at 25 °C is __ kJ/mol.
The equilibrium constants for Reaction 1 and Reaction 2 are 360 and 800, respectively; Gas constant \( R = 8.314 \, {J mol}^{-1} {K}^{-1} \).
(Round off to two decimal places)