Question:
Process used for amplification or multiplication of DNA for finger printing is
Process used for amplification or multiplication of DNA for finger printing is
Updated On: Jun 20, 2022
- Polymerase chain reaction
- Nesslerisation
- Southern blotting
- Northern blotting
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The Correct Option is A
Solution and Explanation
Polymerase Chain Reaction (PCR) technique is a method for making copies of specific stretches of DNA from single cell as well as from DNA libraries. Thus, PCR has allowed the analysis of minute quantities of evidences and has facilitated DNA finger printing.
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.