Question:
Plasmid $pBR322$ has PstI restriction enzyme site within gene $a m p^{R}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain
Plasmid $pBR322$ has PstI restriction enzyme site within gene $a m p^{R}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain
Updated On: May 18, 2024
- it will not be able to confer ampicillin resistance to the host cell.
- the transformed cells will have the ability to resist ampicillin as well as produce $\beta$-galactoside.
- it will lead to lysis of host cell.
- it will be able to produce a novel protein with dual ability.
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The Correct Option is A
Solution and Explanation
Since the gene is inserted at Pst I of $a m p^{R}$ region of pBR322, the $a m p^{R}$ gene is inactivated which is called as insertional inactivation. Hence the genetically modified E.coli strain will not be able to confer ampicillin resistance.
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
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