Question

One of the following is the correct sequence to make a transgenic animal.

• A. Transgenics – transfection – microinjection – electroporation – retroviral vectors
• B. Microinjection – transfection – electroporation – retroviral vectors – transgenics
• C. Transfection – microinjection – transgenics – electroporation – retroviral vectors
• D. None of these

Hint:

Methods like microinjection, electroporation, and retroviral vectors are \textbf{alternative techniques}, not steps in a single sequence.

Answer 1

The Correct Option is D

Step 1: A transgenic animal is one whose genome has been permanently altered by the introduction of a foreign gene.
Step 2: There is no single fixed sequence of steps to produce a transgenic animal.
Step 3: Different independent techniques are used for gene transfer, such as:

Microinjection
Electroporation
Transfection
Retroviral vector-mediated gene transfer

Step 4: These methods are alternative approaches, not sequential steps used one after the other.
Step 5: The term transgenics refers to the \emph{final product}, not a procedural step.
Step 6: Hence, none of the given options correctly represent a valid sequence.