Microsatellites are
- Repetitive DNA sequences
- ESTs
- YAC
- BAC
The Correct Option is A
Solution and Explanation
Top Questions on DNA fingerprinting
- Arrange the given steps of DNA finger printing technique in correct sequence.
A. Isolation of DNA and its digestion by restriction and endonuclease
B. Seperation of DNA fragments by electrophoresis
C. Hybridization with labelled VNTR probe and autoradiography
D. Blotting of seperated DNA fragments to synthetic membrane
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
- The steps of DNA fingerprinting are given below. Arrange them in a correct sequence.
(A)Separation of DNA fragments by electrophoresis
(B)Hybridization using labelled VNTR probe
(C)Isolation and digestion of DNA
(D)Detection of hybridized DNA fragments by autoradiography
(E)Transferring Of separated DNA fragments to synthetic membranes
Choose the correct answer from the options given below :- CUET (UG) - 2023
- Biology
- DNA fingerprinting
Which aspect forms the basis of DNA finger- printing?
- KCET - 2022
- Biology
- DNA fingerprinting
- DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
- DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as:
- NEET (UG) - 2021
- Biology
- DNA fingerprinting
Questions Asked in KEAM exam
- A Spherical ball is subjected to a pressure of 100 atmosphere. If the bulk modulus of the ball is 1011 N/m2,then change in the volume is:
- KEAM - 2023
- mechanical properties of solids
- Two identical solid spheres,each of radius 10cm,are kept in contact. If the mass of inertia of this system about the tangent passing through the point of contact is 0. Then mass of each sphere is:
- KEAM - 2023
- System of Particles & Rotational Motion
- The value of a(≠0) for which the equation \(\frac{1}{2}(x-2)^2+1=\sin(\frac{a}{x})\) holds is/are
- KEAM - 2023
- Trigonometric Functions
- \(∫xlog(1+x^2)dx=\)?
- KEAM - 2023
- Integration by Parts
- A uniform thin rod of mass 3kg has a length of 1m. If a point mass of 1kg is attached to it at a distance of 40cm from its center,the center of mass shifts by a distance of:
- KEAM - 2023
- System of Particles & Rotational Motion
Concepts Used:
DNA Fingerprinting
DNA fingerprinting, also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing, in genetics, method of isolating and identifying variable elements within the base-pair sequence of DNA.
The procedure of DNA Fingerprinting:
The procedure for creating a DNA fingerprint consists of first obtaining a sample of cells, such as skin, hair, or blood cells, which contain DNA. The DNA is extracted from the cells and purified. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific points along the strand with proteins known as restriction enzymes. The enzymes produced fragments of varying lengths that were sorted by placing them on a gel and then subjecting the gel to an electric current (electrophoresis): the shorter the fragment, the more quickly it moved toward the positive pole (anode). The sorted double-stranded DNA fragments were then subjected to a blotting technique in which they were split into single strands and transferred to a nylon sheet. The fragments underwent autoradiography in which they were exposed to DNA probes—pieces of synthetic DNA that were made radioactive and that bound to the minisatellites. A piece of X-ray film was then exposed to the fragments, and a dark mark was produced at any point where a radioactive probe had become attached. The resultant pattern of marks could then be analyzed.