Plasmids can vary in size, but the maximum number of bases in plasmids discovered so far can exceed 500 kilobases (kb) and can even reach into the megabase (Mb) range for some exceptionally large plasmids. However, it's important to note that plasmids can be smaller or larger depending on the specific plasmid and its role in carrying genetic information in various organisms. So, the closest option is (B): 500 kilobases (kb)
Answer the following questions:
(a) [(i)] Explain how some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans but do not kill the Bacillus.
[(ii)] How is the above mechanism exploited for the production of Bt cotton plant by biotechnologists?
(b) [(i)] Explain how the amplification of gene of interest is done using PCR.
[(ii)] State two applications of the desired amplified fragment of DNA.
The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow.
(a) What is the technical term used for Step 4 in the above process?
(b) Which of the given two combinations of restriction enzyme should be used in Step 1? Justify your answer.
(i) EcoR I to cut the plasmid and Hind III to cut the alien DNA.
(ii) EcoR I to cut both the plasmid and alien DNA.
The current passing through the battery in the given circuit, is:
A bob of heavy mass \(m\) is suspended by a light string of length \(l\). The bob is given a horizontal velocity \(v_0\) as shown in figure. If the string gets slack at some point P making an angle \( \theta \) from the horizontal, the ratio of the speed \(v\) of the bob at point P to its initial speed \(v_0\) is :
A full wave rectifier circuit with diodes (\(D_1\)) and (\(D_2\)) is shown in the figure. If input supply voltage \(V_{in} = 220 \sin(100 \pi t)\) volt, then at \(t = 15\) msec:
Acid is any hydrogen-containing substance that is capable of donating a proton (hydrogen ion) to another substance. Base is an ion or molecule capable of accepting a hydrogen ion from acid.
Physical Properties | ACIDS | BASES |
Taste | Sour | Bitter |
Colour on Litmus paper | Turns blue litmus red | Turns red litmus blue |
Ions produced on dissociation | H+ | OH- |
pH | <7 (less than 7) | >7 (more than 7) |
Strong acids | HCl, HNO3, H2SO4 | NaOH, KOH |
Weak Acids | CH3COOH, H3PO4, H2CO3 | NH4OH |
Type of Reaction | Acid | Bases |
Reaction with Metals | Acid + Metal → Salt + Hydrogen gas (H2) E.g., Zn(s)+ dil. H2SO4 → ZnSO4 (Zinc Sulphate) + H2 | Base + Metal → Salt + Hydrogen gas (H2) E.g., 2NaOH +Zn → Na2ZnO2 (Sodium zincate) + H2 |
Reaction with hydrogen carbonates (bicarbonate) and carbonates | Metal carbonate/Metal hydrogen carbonate + Acid → Salt + Carbon dioxide + Water E.g., HCl+NaOH → NaCl+ H2O 2. Na2CO3+ 2 HCl(aq) →2NaCl(aq)+ H2O(l) + CO2(g) 3. Na2CO3+ 2H2SO4(aq) →2Na2SO4(aq)+ H2O(l) + CO2(g) 4. NaHCO3+ HCl → NaCl+ H2O+ CO2 | Base+ Carbonate/ bicarbonate → No reaction |
Neutralisation Reaction | Base + Acid → Salt + Water E.g., NaOH(aq) + HCl(aq) → NaCl(aq) + H2O(l) | Base + Acid → Salt + Water E.g., CaO+ HCl (l) → CaCl2 (aq)+ H2O (l) |
Reaction with Oxides | Metal oxide + Acid → Salt + Water E.g., CaO+ HCl (l) → CaCl2 (aq)+ H2O (l) | Non- Metallic oxide + Base → Salt + Water E.g., Ca(OH)2+ CO2 → CaCO3+ H2O |
Dissolution in Water | Acid gives H+ ions in water. E.g., HCl → H+ + Cl- HCl + H2O → H3O+ + Cl– | Base gives OH- ions in water. |
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