Question

Match LIST-I with LIST-II

Choose the correct answer from the options given below:

• A. A - IV, B - III, C - I, D - II
• B. A - IV, B - III, C - II, D - I
• C. A - I, B - II, C - IV, D - III
• D. A - III, B - IV, C - I, D - II

Hint:

Remember the key players in E. coli replication: DnaA (initiator), DnaB (Helicase), DnaC (loader), DnaG (Primase), and Pol III (main polymerase). Pol I (`polA`) is famous for primer removal, and Pol II (`polB`) is for repair.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
This question requires matching the genes involved in E. coli DNA replication (LIST-I) with their corresponding protein products or roles (LIST-II).
Step 2: Detailed Explanation:
Let's match each gene with its product/role:


A. polA: This gene codes for DNA polymerase I, an enzyme known for its role in DNA repair and removing RNA primers. This matches IV. DNA polymerase I.

B. polB: This gene codes for DNA polymerase II, an enzyme primarily involved in DNA repair. This matches III. DNA polymerase II.

C. DnaG: This gene codes for the enzyme Primase, which synthesizes short RNA primers to initiate DNA synthesis. This matches II. Primase.

D. DnaB \& C: DnaB is the main replicative Helicase in E. coli, which unwinds the DNA double helix. DnaC is the helicase loader, which helps place DnaB at the origin of replication (OriC). This complex is therefore responsible for the helicase activity at the origin. This matches I. Helicase at OriC.

Step 3: Final Answer:
The correct matching is: A-IV, B-III, C-II, D-I. This corresponds to option (B).