Match Group-I (selection agent) and Group-II (gene) in CORRECT combination.
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Understanding the relationship between selection agents and their corresponding resistance genes is key in genetic engineering and plant transformation.
Step 1: Understanding the selection agents and their associated genes.
- Kanamycin is an antibiotic selection agent, and the corresponding gene is \( nptII \) (neomycin phosphotransferase), which confers resistance to kanamycin. This corresponds to P-III.
- Hygromycin is another antibiotic, and the corresponding gene is \( hpt \), which confers resistance to hygromycin. This corresponds to Q-VI.
- Phosphinothricin is an herbicide, and the corresponding gene is \( bar \), which provides resistance to phosphinothricin. This corresponds to R-II.
- Mannose is a sugar, and the gene associated with it is \( pmi \), which provides resistance to mannose. This corresponds to S-I.
Step 2: Analyzing the options.
- (A) P-III, Q-VI, R-II, S-I: Correct, as this matches the proper selection agent to gene pairings.
- (B) P-IV, Q-III, R-II, S-I: Incorrect, as the pairing for P-IV and Q-III does not match.
- (C) P-I, Q-VI, R-III, S-II: Incorrect, as P-I and R-III do not match.
- (D) P-II, Q-I, R-V, S-VI: Incorrect, as these pairings do not match the correct selection agents and genes.
Step 3: Conclusion.
The correct answer is (A), as the matching of selection agents with the appropriate genes is accurate.
