Question

It is not involved in recombinant DNA production.

• A. Restriction endonuclease
• B. DNA ligase
• C. DNA fragments
• D. E. coli

Hint:

\textit{E. coli} is used as a host to express recombinant DNA, not in its creation.

Answer 1

The Correct Option is D

The process of recombinant DNA technology involves the following key components:
- Restriction endonucleases: Enzymes used to cut DNA at specific sequences.
- DNA ligase: Enzyme that joins DNA fragments to form recombinant DNA.
- DNA fragments: Segments of interest (gene of interest) used for recombination.
However, \textit{E. coli} is not involved in the production of recombinant DNA — it is typically used after production, as a host organism to clone or express the recombinant DNA.
Thus, while \textit{E. coli} plays a role in cloning or transformation steps, it is not directly involved in the actual formation of recombinant DNA molecules.

Answer 2

It is not involved in recombinant DNA production:

Recombinant DNA technology involves the manipulation of DNA sequences to create new genetic combinations that are not found naturally. Various organisms, enzymes, and tools play key roles in this process.

E. coli is a commonly used host organism for cloning and expressing recombinant DNA, but it is not directly involved in the production or creation of recombinant DNA itself. Rather, it serves as a vehicle to replicate or express the recombinant DNA once it has been constructed.

The actual production of recombinant DNA involves enzymes like restriction endonucleases (to cut DNA), ligases (to join DNA fragments), and vectors (plasmids) to carry the recombinant DNA.

Thus, E. coli is essential for cloning and propagation but not for the initial production of recombinant DNA.

Correct Answer: E. coli