Question

Identify A, B, C and D in the diagram of E.coli cloning vector of pBR322:

Hint:

Memorize common restriction sites on pBR322.
AmpR and TetR = selectable markers.
Ori = origin of replication.

Answer 1

1. pBR322 map: Hind I → site A, EcoRI → site B, ampR → antibiotic resistance, Ori → origin of replication.
2. BamHI & KanR are different sites/genes.
3. Correct identification is (1) A-Hind I, B-ECoR1, C-ampR, D-Ori.