Question

In the microbial bioassay of Amikacin, the strain used is \ldots

• A. Bacillus pumilus
• B. Saccharomyces cerevisiae
• C. Bacillus subtilis
• D. Staphylococcus aureus

Hint:

Microbial bioassays measure antibiotic potency by observing the growth inhibition of a sensitive bacterial strain.

Answer 1

The Correct Option is D

Amikacin is an aminoglycoside antibiotic, and its potency is often assessed using a microbial bioassay. In this assay, Staphylococcus aureus is commonly used as the test organism because it is sensitive to Amikacin, allowing for accurate measurement of the drug’s antibacterial activity through inhibition zones. Bacillus pumilus, Saccharomyces cerevisiae (a yeast), and Bacillus subtilis are not typically used for Amikacin bioassays.
Thus, the correct answer is Staphylococcus aureus.