Step 1: Understanding testis temperature regulation.
The testis needs to be kept at a temperature lower than the core body temperature for optimal sperm production. This is achieved by the scrotum, which hangs outside the body to provide a cooler environment.
Step 2: Analyzing the options.
(A) proximal tubule: Incorrect — The proximal tubule is part of the kidney, not involved in regulating testis temperature.
(B) loop of Henle: Incorrect — The loop of Henle is involved in kidney function, not temperature regulation of the testis.
(C) scrotum: Correct — The scrotum holds the testes outside the body, which helps in maintaining a lower temperature.
(D) seminal vesicles: Incorrect — Seminal vesicles produce seminal fluid, not involved in temperature regulation of the testes.
Step 3: Conclusion.
The correct answer is (C) scrotum.
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 