Question

In HPLC, when the packing material used in the column is polar in nature and the mobile phase is relatively non-polar, it is known as:

• A. Normal phase
• B. Reverse phase
• C. Neutral phase
• D. Stationary phase

Hint:

Understand phase polarity in HPLC to choose the correct method for analyzing chemical mixtures.

Answer 1

The Correct Option is A

Normal phase chromatography utilizes a polar stationary phase and a non-polar mobile phase. It is primarily used for separating compounds based on their polarity, with polar compounds being retained longer due to their stronger interaction with the stationary phase.