Question

In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?
P. Alkaline phosphatase
Q. Trypsinase
R. Horseradish peroxidase
S. Amylase

• A. P and R
• B. P and Q
• C. Q and S
• D. R and S

Hint:

- ELISA uses enzyme-antibody conjugates for detection.
- HRP and AP are the standard enzymes because of high stability and chromogenic substrates.
- Avoid confusion with digestive enzymes like amylase or trypsin.

Answer 1

The Correct Option is A

Step 1: Enzyme-linked immunosorbent assay (ELISA) uses enzyme-conjugated antibodies to detect antigen-antibody binding.
Step 2: The most commonly used enzymes are:
- Alkaline phosphatase (AP)
- Horseradish peroxidase (HRP)
Step 3: Trypsinase and amylase are not used in ELISA because they are not suitable for sensitive chromogenic/fluorogenic detection.
Final Answer: The enzymes used are Alkaline phosphatase and Horseradish peroxidase → option (A).
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