Question:
In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?
P. Alkaline phosphatase
Q. Trypsinase
R. Horseradish peroxidase
S. Amylase
In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?
P. Alkaline phosphatase
Q. Trypsinase
R. Horseradish peroxidase
S. Amylase
P. Alkaline phosphatase
Q. Trypsinase
R. Horseradish peroxidase
S. Amylase
Show Hint
- ELISA uses enzyme-antibody conjugates for detection.
- HRP and AP are the standard enzymes because of high stability and chromogenic substrates.
- Avoid confusion with digestive enzymes like amylase or trypsin.
- HRP and AP are the standard enzymes because of high stability and chromogenic substrates.
- Avoid confusion with digestive enzymes like amylase or trypsin.
Updated On: Aug 26, 2025
- P and R
- P and Q
- Q and S
- R and S
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The Correct Option is A
Solution and Explanation
Step 1: Enzyme-linked immunosorbent assay (ELISA) uses enzyme-conjugated antibodies to detect antigen-antibody binding.
Step 2: The most commonly used enzymes are:
- Alkaline phosphatase (AP)
- Horseradish peroxidase (HRP)
Step 3: Trypsinase and amylase are not used in ELISA because they are not suitable for sensitive chromogenic/fluorogenic detection.
Final Answer: The enzymes used are Alkaline phosphatase and Horseradish peroxidase → option (A).
\[\boxed{\text{Correct Answer: (A)}}\]
Step 2: The most commonly used enzymes are:
- Alkaline phosphatase (AP)
- Horseradish peroxidase (HRP)
Step 3: Trypsinase and amylase are not used in ELISA because they are not suitable for sensitive chromogenic/fluorogenic detection.
Final Answer: The enzymes used are Alkaline phosphatase and Horseradish peroxidase → option (A).
\[\boxed{\text{Correct Answer: (A)}}\]
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