Question

(ii) State the role of ‘rop’ gene.

Hint:

The rop gene ensures controlled plasmid replication, essential for stable cloning.

Answer 1

The rop gene in pBR322 regulates plasmid replication, maintaining a low copy number for stable maintenance. Key functions include: - Preventing host cell burden: High plasmid copy numbers can stress the host cell, reducing its growth and efficiency. - Modulating replication: Rop regulates RNA molecules involved in plasmid replication, ensuring balance. This gene is critical for maintaining the integrity of inserted foreign DNA during cloning experiments. % Quick tip