Question

(ii) Name the vector that is used to introduce nematode-specific genes into the host plant (tobacco).

Hint:

Agrobacterium tumefaciens is a natural genetic engineer widely used in transgenic research.

Answer 1

The vector used is Agrobacterium tumefaciens. Key Features: \begin{itemize} \item Ti Plasmid: The bacterium contains a tumor-inducing (Ti) plasmid, modified to include nematode-specific genes for safe transfer. \item RNAi Technology: The introduced genes produce double-stranded RNA, which triggers RNA interference (RNAi) to silence critical nematode genes. \item Eco-friendly Protection: Transgenic tobacco plants resist nematode infestation without the need for chemical pesticides. \end{itemize} Applications: \begin{itemize} \item Agrobacterium-mediated gene transfer is widely used to create transgenic plants with improved traits, such as pest and disease resistance. \end{itemize} % Quick tip