Question

Identify the incorrect statement regarding PCR.

• A. Two sets of primers are required during polymerisation.
• B. The process of replication is repeated multiple times to produce one billion copies.
• C. Thermostable DNA polymerase is used for extension of primers.
• D. Annealing is required to separate both the strands of template DNA.

Hint:

PCR steps: Denaturation (strand separation), Annealing (primer binding), Extension (DNA synthesis).

Answer 1

The Correct Option is D

Annealing is the step where primers bind to single-stranded DNA after denaturation.
Strand separation is achieved during the denaturation step (usually at 94–96°C), not during annealing.