Question

(i) Draw a schematic diagram of the cloning vector pBR322 and label:
(1) BamHI site 
(2) Gene for ampicillin resistance 
(3) Ori 
(4) Rop gene 

Hint:

pBR322 is one of the first widely used cloning vectors in genetic engineering.

Answer 1

The pBR322 vector is a widely used plasmid in genetic engineering. Key features include:
 - BamHI site: A restriction site for inserting foreign DNA. 
- Ampicillin resistance gene: Enables selection of transformed cells that can grow in the presence of ampicillin. 
- Ori (Origin of replication): Ensures plasmid replication within the host cells. 
- Rop gene: Regulates plasmid copy number for stable cloning. These components make pBR322 an efficient and versatile vector for molecular cloning, simplifying the process of inserting and propagating foreign DNA in host cells.