Question:
From the colligative properties of solution, which one is the best method for the determination of molecular weight of proteins and polymers?
From the colligative properties of solution, which one is the best method for the determination of molecular weight of proteins and polymers?
Updated On: May 4, 2024
- Osmotic pressure
- Lowering in vapour pressure
- Lowering in freezing point
- Elevation in boiling point
Hide Solution
Verified By Collegedunia
The Correct Option is A
Solution and Explanation
Osmotic pressure is the best method for the determination of molar mass of proteins and polymers.Because a small amount of solute will produce a much larger change in this quantity than in the boiling point, freezing point, or vapor pressure. even a 106 molar solution would have a measurable osmotic pressure. Molecular weight determinations are very frequently made on proteins or other high molecular weight polymers. These substances, owing to their large molecular size, tend to be only sparingly soluble in most solvents, so measurement of osmotic pressure is often the only practical way of determining their molecular weights.
Was this answer helpful?
0
0
Top Questions on Solutions
- The spontaneous flow of the solvent through a semipermeable membrane from a pure solvent to a solution or from a dilute solution to a concentrated solution is called osmosis. The phenomenon of osmosis can be demonstrated by taking two eggs of the same size. In an egg, the membrane below the shell and around the egg material is semipermeable. The outer hard shell can be removed by putting the egg in dilute hydrochloric acid. After removing the hard shell, one egg is placed in distilled water and the other in a saturated salt solution. After some time, the egg placed in distilled water swells up while the egg placed in salt solution shrinks. The external pressure applied to stop the osmosis is termed as osmotic pressure (a colligative property). Reverse osmosis takes place when the applied external pressure becomes larger than the osmotic pressure.
- A solution containing 15 g urea (molar mass = 60 g mol\(^{-1}\)) per litre of solution in water has the same osmotic pressure (isotonic) as a solution of glucose (molar mass = 180 g mol\(^{-1}\)) in water. Calculate the mass of glucose present in one litre of its solution.
- Define Azeotrope. What type of Azeotrope is formed by negative deviation from Raoult's law? Give an example.
- A solution of glucose (molar mass = 180 g mol\(^{-1}\)) in water has a boiling point of 100.20°C. Calculate the freezing point of the same solution. Molal constants for water \(K_f\) and \(K_b\) are 1.86 K kg mol\(^{-1}\) and 0.512 K kg mol\(^{-1}\) respectively.
- An unripe mango placed in a concentrated salt solution to prepare pickle, shrivels because __________
View More Questions
Questions Asked in NEET exam
- In an ecosystem if the Net Primary Productivity (NPP) of first trophic level is 100x (kcal m-2)yr-1, what would be the GPP (Gross Primary Productivity) of the third trophic level of the same ecosystem?
- NEET (UG) - 2024
- Ecosystems
- A light ray enters through a right angled prism at point P with the angle of incidence 30° as shown in figure. It travels through the prism parallel to its base BC and emerges along the face AC. The refractive index of the prism is :
- NEET (UG) - 2024
- Refraction Through A Prism
- Match List I with List II:Choose the correct answer from the options given below:
List I
List II
A Mesozoic Era I Lower invertebrates B Proterozoic Era II Fish & Amphibia C Cenozoic Era III Birds & Reptiles D Paleozoic Era IV Mammals - NEET (UG) - 2024
- Origin Of Life
- \(^{290}_{82}X\stackrel{\alpha}{\rightarrow}Y\stackrel{e^+}{\rightarrow}Z\stackrel{\beta^-}{\rightarrow}P\stackrel{e^-}{\rightarrow}Q\)
In the nuclear emission stated above, the mass number and atomic number of the product Q respectively, are :- NEET (UG) - 2024
- Nuclear physics
- In a plant, black seed color (BB/Bb) is dominant over white seed color (bb). In order to find out the genotype of the black seed plant, with which of the following genotype will you cross it?
- NEET (UG) - 2024
- monohybrid cross
View More Questions
Concepts Used:
Solutions
A solution is a homogeneous mixture of two or more components in which the particle size is smaller than 1 nm.
For example, salt and sugar is a good illustration of a solution. A solution can be categorized into several components.
Types of Solutions:
The solutions can be classified into three types:
- Solid Solutions - In these solutions, the solvent is in a Solid-state.
- Liquid Solutions- In these solutions, the solvent is in a Liquid state.
- Gaseous Solutions - In these solutions, the solvent is in a Gaseous state.
On the basis of the amount of solute dissolved in a solvent, solutions are divided into the following types:
- Unsaturated Solution- A solution in which more solute can be dissolved without raising the temperature of the solution is known as an unsaturated solution.
- Saturated Solution- A solution in which no solute can be dissolved after reaching a certain amount of temperature is known as an unsaturated saturated solution.
- Supersaturated Solution- A solution that contains more solute than the maximum amount at a certain temperature is known as a supersaturated solution.