During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out
DNA
Histones
Polysaccharides
RNA
The Correct Option is A
Solution and Explanation
In recombinant DNA technology, when chilled ethanol is added to the solution containing the DNA of interest, the DNA molecules become insoluble and form a visible white precipitate. This occurs because DNA is not soluble in cold ethanol. This precipitation step allows the separation and purification of DNA from other cellular components and contaminants in the sample.
Therefore, The correct option is (A): DNA
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Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.