Question:
During the process of isolation of DNA, chilled ethanol is added to :
During the process of isolation of DNA, chilled ethanol is added to :
Updated On: May 3, 2024
- Precipitate DNA
- Break open the cell to release DNA
- Facilitate action of restriction enzymes
- Remove proteins such as histones
Hide Solution
Verified By Collegedunia
The Correct Option is A
Solution and Explanation
Ethanol is much less polar than water. Adding it to the solution disrupts the screening charges exerted by water. The electrical attraction between phosphate and any positive ions (Na+)present in solution becomes strong enough to form a stable ionic bond and DNA precipitates. Ethanol precipitation is a widely used technique to purify, or concentrate nucleic acid.
Was this answer helpful?
0
0
Top Questions on Biotechnology
- In recombinant DNA technology, which enzyme is used to cut DNA at specific recognition sites to produce restriction fragments?
- MHT CET - 2025
- Biology
- Biotechnology
- Combination of foreign DNA and vector DNA are together called as _______.
- MHT CET - 2025
- Biology
- Biotechnology
- In genetic engineering the Foreign DNA is called
- MHT CET - 2025
- Biology
- Biotechnology
- How many time needed for PCR?
- MHT CET - 2025
- Biology
- Biotechnology
- The picture given below shows:
(a) Roots of a typical control tobacco crop plant (infected).
(b) Transgenic tobacco plant showing healthy roots even after deliberate infection by nematode.
Explain how this transformation was achieved in the tobacco plant.- CBSE CLASS XII - 2024
- Biology
- Biotechnology
View More Questions
Questions Asked in NEET exam
- Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Cells of the tapetum possess dense cytoplasm and generally have more than one nucleus.
Reason (R): Presence of more than one nucleus in the tapetum increases the efficiency of nourishing the developing microspore mother cells.- NEET (UG) - 2025
- Reproduction in Plants
- Given below are two statements:
Statement I: Fig fruit is a non-vegetarian fruit as it has enclosed fig wasps in it.
Statement II: Fig wasp and fig tree exhibit a mutual relationship as the fig wasp completes its life cycle in the fig fruit and the fig fruit gets pollinated by the fig wasp. A full wave rectifier circuit with diodes (\(D_1\)) and (\(D_2\)) is shown in the figure. If input supply voltage \(V_{in} = 220 \sin(100 \pi t)\) volt, then at \(t = 15\) msec:
- NEET (UG) - 2025
- Semiconductor Diode
- Two cities X and Y are connected by a regular bus service with a bus leaving in either direction every T min. A girl is driving scooty with a speed of 60 km/h in the direction X to Y. She notices that a bus goes past her every 30 minutes in the direction of her motion, and every 10 minutes in the opposite direction. Choose the correct option for the period T of the bus service and the speed (assumed constant) of the buses.
- NEET (UG) - 2025
- Relative Velocity
- An AC power supply of 220 V at 50 Hz, a resistor of 20\( \Omega \), a capacitor of reactance 25\( \Omega \) and an inductor of reactance 45\( \Omega \) are connected in series. The corresponding current in the circuit and the phase angle between the current and the voltage is, respectively :
- NEET (UG) - 2025
- Alternating Current
View More Questions
Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.