Question:
During the process of isolation of DNA, chilled ethanol is added to :
During the process of isolation of DNA, chilled ethanol is added to :
Updated On: May 3, 2024
- Precipitate DNA
- Break open the cell to release DNA
- Facilitate action of restriction enzymes
- Remove proteins such as histones
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The Correct Option is A
Solution and Explanation
Ethanol is much less polar than water. Adding it to the solution disrupts the screening charges exerted by water. The electrical attraction between phosphate and any positive ions (Na+)present in solution becomes strong enough to form a stable ionic bond and DNA precipitates. Ethanol precipitation is a widely used technique to purify, or concentrate nucleic acid.
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Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.