Do eukaryotic cells have restriction endonucleases? Justify your answer.
Solution and Explanation
No, eukaryotic cells do not have restriction endonucleases. This is because the DNA of eukaryotes is highly methylated by a modification enzyme, called methylase. Methylation protects the DNA from the activity of restriction enzymes. These enzymes are present in prokaryotic cells where they help prevent the invasion of DNA by virus.
Top Questions on Biotechnology
- Which enzyme is used to join two DNA fragments in genetic engineering?
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- Identify the incorrect statement related to the following context:
The model experiment for assessing certain types of genetically modified animals can be conducted.
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Questions Asked in CBSE CLASS XII exam
A ladder of fixed length \( h \) is to be placed along the wall such that it is free to move along the height of the wall.
Based upon the above information, answer the following questions:(iii) (b) If the foot of the ladder, whose length is 5 m, is being pulled towards the wall such that the rate of decrease of distance \( y \) is \( 2 \, \text{m/s} \), then at what rate is the height on the wall \( x \) increasing when the foot of the ladder is 3 m away from the wall?
- CBSE CLASS XII - 2025
- Application of derivatives
- A meeting will be held only if all three members A, B and C are present. The probability that member A does not turn up is 0.10, member B does not turn up is 0.20 and member C does not turn up is 0.05. The probability of the meeting being cancelled is:
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- Probability
- Vishesh, Manik and Amit were partners in the ratio 5 : 4 : 1. Amit retired on 31st March, 2024. Vishesh and Manik decided to share Amit’s share in the ratio 2 : 3. What will be the new profit sharing ratio between Vishesh and Manik?
- CBSE CLASS XII - 2025
- Retirement and Death of a Partner
- The electric field in a region is given by \( \vec{E} = 40x \hat{i} \, \text{N/C}. \) Find the amount of work done in taking a unit positive charge from a point (0, 3m) to the point (5m, 0).
- CBSE CLASS XII - 2025
- Electrostatics
- Consider the Linear Programming Problem, where the objective function \[ Z = x + 4y \] needs to be minimized subject to the following constraints: \[ 2x + y \geq 1000, \] \[ x + 2y \geq 800, \] \[ x \geq 0, \quad y \geq 0. \] Draw a neat graph of the feasible region and find the minimum value of $Z$.
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- Linear Programming Problem
Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.