Chordates and non-chordates can be distinguished by the presence or absence of key structural features at some stage of development. Major distinguishing characters are listed below.
| Feature | Chordates ; Non-chordates |
| Notochord | Present at some stage ; Absent |
| Dorsal hollow nerve cord | Present ; Usually ventral solid nerve cord or diffuse nerve net |
| Pharyngeal gill slits | Present at some stage ; Absent or modified differently |
| Post-anal tail | Present at some stage ; Absent or not persistent |
| Segmental arrangement | Myotomes and segmented muscles usually present ; Segmentation variable or absent |
| Endoskeleton | Often present (cartilage or bone) ; Usually absent or present as exoskeleton |
| Circulatory system | Closed in many chordates ; Usually open or more variable in non-chordates |
| Cephalization and advanced organ systems | Well developed (brain, sensory organs) ; Less specialized in many non-chordates |
Summary statement: Chordates are defined by the notochord, dorsal hollow nerve cord, pharyngeal slits, and post-anal tail appearing at some stage of life, whereas non-chordates lack this specific combination of features and show greater diversity in body plans and organ-system organization.