Question

Culture media used for E.coli 0157:H7 is

• A. SMAC
• B. Wilson and Blair medium
• C. Potassium tellurite in Mcleod's medium
• D. Deoxycholate citrate agar (DCA)

Answer 1

The Correct Option is A

To identify the culture media used for E.coli O157:H7, it's important to understand the role of each option provided:

1. SMAC: Sorbitol MacConkey Agar is specifically designed for detecting E.coli O157:H7. While most E.coli strains can ferment sorbitol, E.coli O157:H7 cannot, resulting in colorless colonies which helps in its identification.
2. Wilson and Blair Medium: This is typically used for the isolation of Salmonella and not suitable for E.coli O157:H7.
3. Potassium Tellurite in Mcleod's Medium: Often used for isolating Corynebacteria, and not relevant for E.coli O157:H7 detection.
4. Deoxycholate Citrate Agar (DCA): Primarily used for isolating Enterobacteriaceae, particularly Salmonella and Shigella, but not for E.coli O157:H7.

Based on this analysis, the appropriate culture medium for isolating and identifying E.coli O157:H7 is SMAC.