Question

Complete the diagram showing various processes in tissue culture 

Hint:

Key stages to fill diagram blanks: Explant selection, Surface sterilization, Inoculation on medium, and Hardening/acclimatization before field planting.

Answer 1

1. Explant selection and excision: Choose a healthy part (meristem/shoot tip) from the mature plant and excise it aseptically.
2. Surface sterilization: Remove surface microbes from the explant using disinfectants so it can be handled in sterile conditions.
3. Inoculation on sterile culture medium: Place the sterilized explant on nutrient agar or liquid medium with growth regulators to induce callus formation and shoot initiation.
4. Shooting and Rooting: Promote shoot multiplication and root formation using appropriate hormones in vitro.
5. Hardening and acclimatization: Gradually adapt rooted plantlets to external conditions by transferring them to soil/greenhouse under controlled humidity and light.
6. Planting and maturation: Transplant hardened plantlets to the field or nursery where they grow into mature plants, completing the cycle.