Question

Choose the correct statement(s) about the enzyme and its application in food processing reaction.

• A. Chymosin is widely used in cheese manufacturing.
• B. Thermolysin is used in the synthesis of Aspartame.
• C. $\beta$-Galactosidase catalyzes the hydrolysis of galactose.
• D. Lipase is used for restructuring of acyl glycerol.

Hint:

Remember: - Chymosin → cheese, - Thermolysin → Aspartame, - $\beta$-Galactosidase → lactose hydrolysis, - Lipase → fat restructuring.

Answer 1

The Correct Option is A, B, D

Step 1: Chymosin. This enzyme (rennin) is widely used in cheese manufacturing because it specifically cleaves $\kappa$-casein, leading to milk coagulation. Hence, (A) is correct.
Step 2: Thermolysin. A thermostable protease used industrially in the synthesis of Aspartame (a low-calorie artificial sweetener). Hence, (B) is correct.
Step 3: $\beta$-Galactosidase. This enzyme hydrolyzes lactose (into glucose and galactose), not galactose itself. So statement (C) is incorrect.
Step 4: Lipase. Lipases are used in the restructuring and modification of fats (acyl glycerols), such as in producing transesterified fats for food applications. Hence, (D) is correct.
Therefore, the correct statements are (A), (B), and (D).