Can you recall meiosis and indicate at what stage a recombinant DNA is made?
Solution and Explanation
Meiosis is a process that involves the reduction in the amount of genetic material. It is two types, namely meiosis I, and meiosis II. During the pachytene stage of prophase I, crossing over the chromosomes takes place where the exchange of segments between non-sister chromatids of homologues chromosomes takes place. This results in the formation of recombinant of DNA.
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Concepts Used:
Recombinant Technology
Recombinant DNA technology is the process used for producing new genetic combinations by joining different genetic material (DNA) together and inserting them into host organisms from two different species or sources. These new combinations are of value to medicine, science, industry, and agriculture.
Process of Recombinant DNA Technology:
Step 1- Isolation of Genetic Material.
Step 2- Cutting the gene at the recognition sites.
Step 3- Amplifying the gene copies through Polymerase chain reaction ( PCR)
Step 4- Ligation of DNA Molecules.
Step 5- Insertion of Recombinant DNA into Host.
Application of Recombinant DNA Technology:
- In agricultural fields Recombinant DNA Technology plays a major role. It produces genetically-modified organisms such as flavor save tomatoes, golden rice rich in protein and lot more
- Recombinant DNA technology is also used to produce Insulin.
- ELISA is one kind of clinical diagnosis where recombinant DNA technology is used.
- Recombinant DNA technology prevents hereditary diseases through gene therapy and also detects the presence of HIV in a person.