Question

Assertion (A): Primers are used in polymerase chain reaction technique. Reason (R): Primers is used for the addition of new DNA nucleotides.

• A. A and R are correct. R is the correct explanation of A
• B. A and R are correct. R is not the correct explanation of A
• C. A is correct but R is incorrect
• D. A is incorrect but R is correct

Hint:

Understanding each component's function in PCR is crucial for designing effective and specific genetic experiments.

Answer 1

The Correct Option is C

To solve the problem, we need to assess the assertion and reason regarding the use of primers in the polymerase chain reaction (PCR) technique.

1. Understanding the Assertion and Reason:
- Assertion (A): Primers are used in the polymerase chain reaction technique.
- Reason (R): Primers are used for the addition of new DNA nucleotides.

2. Analyzing the Assertion (A):
The assertion is correct. In the polymerase chain reaction (PCR), primers are short sequences of nucleotides that provide a starting point for DNA synthesis. They are essential for initiating the process of DNA replication by the DNA polymerase enzyme.

3. Analyzing the Reason (R):
The reason is incorrect. While primers are indeed required in PCR, their role is not to "add new DNA nucleotides." Instead, primers serve as the binding sites for the DNA polymerase to begin DNA synthesis, allowing the enzyme to add nucleotides in the 5' to 3' direction. The actual addition of nucleotides is performed by the DNA polymerase enzyme.

4. Conclusion:
Although the assertion is correct, the reason does not accurately explain why primers are used in PCR. Hence, the correct option is:

Final Answer:
The correct option is (C) A is correct but R is incorrect.