Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Polymerase chain reaction is used in DNA amplification.
Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements, choose the correct answer from the options given below:
Assertion (A): Polymerase chain reaction is used in DNA amplification.
Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements, choose the correct answer from the options given below:
- Both (A) and (R) are correct and (R) is the correct explanation of (A)
- Both (A) and (R) are correct but (R) is not the correct explanation of (A)
- (A) is correct but (R) is not correct
- (A) is not correct but (R) is correct
The Correct Option is B
Solution and Explanation
Assertion (A): Polymerase chain reaction is used in DNA amplification.
The Polymerase Chain Reaction (PCR) is a fundamental technique in molecular biology that allows for the amplification of a specific segment of DNA. By repeatedly denaturing, annealing, and extending the DNA in cycles, it can create millions of copies of the target DNA sequence. Thus, this statement is correct.
Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation.
Selectable markers are genes introduced into a cell to signify the presence of linked genetic material. In genetic engineering, the ampicillin resistance gene is commonly used to determine if a transformation was successful, as only those cells with the resistance will grow in the presence of ampicillin. Therefore, this statement is also correct.
However, while both statements are correct, the Reason (R) provided is not the explanation for Assertion (A). PCR and DNA amplification are processes related to DNA replication in vitro, while the use of an ampicillin resistant gene as a selectable marker pertains to assessing transformation success in gene cloning experiments. Therefore, these are distinct concepts and not explanatory of one another.
Based on this analysis, the correct answer is: Both (A) and (R) are correct but (R) is not the correct explanation of (A).
Top Questions on The DNA
- According to Chargaff's Rule, in a DNA molecule, the amount of adenine (A) is always equal to the amount of ______.
- The DNA present in chloroplast is:
- Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
- What is the fate of a piece of DNA carrying only gene of interest which is transfered in to an alien organism?
A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiple and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromasame.
E. It shows ability to replicate.
Choose the correct answer from the options given below: - Who discovered DNA?
Concepts Used:
discovery of dna
The discovery of DNA (deoxyribonucleic acid) as the genetic material of living organisms is a significant milestone in the field of biology. The discovery of DNA can be attributed to several scientists whose work collectively contributed to our understanding of its structure and function.
In the early 20th century, several researchers began investigating the nature of inheritance and the transfer of genetic traits. One important figure in this field was Gregor Mendel, an Austrian monk who conducted groundbreaking experiments with pea plants in the 1860s, laying the foundation for the study of genetics.
In the early 1950s, James Watson and Francis Crick, along with Rosalind Franklin and Maurice Wilkins, made the breakthrough discovery of the structure of DNA. Their work was primarily based on X-ray crystallography images obtained by Franklin and Wilkins, which hinted at the helical structure of DNA.
Using Franklin's data and their own modeling experiments, Watson and Crick proposed the double-helix structure of DNA in 1953. They suggested that DNA is composed of two complementary strands wound around each other in a spiral staircase-like structure, with the bases adenine (A) pairing with thymine (T), and guanine (G) pairing with cytosine (C).
This discovery provided insights into how genetic information is stored and transmitted from one generation to the next. It paved the way for a better understanding of DNA replication, protein synthesis, and the role of DNA in inheritance and evolution.
Watson, Crick, and Wilkins were awarded the Nobel Prize in Physiology or Medicine in 1962 for their discoveries related to the structure of DNA. Unfortunately, Franklin, who made significant contributions to the field, passed away in 1958 and was therefore ineligible for the Nobel Prize.
The discovery of DNA revolutionized biology and has had far-reaching implications in various fields, including medicine, agriculture, forensics, and biotechnology. It has led to advancements in gene sequencing, genetic engineering, and the understanding of genetic diseases, ultimately shaping our knowledge of life itself.